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Biotinylated Mouse SPINT1 Protein, His,Avitag™ (MALS verified)

  • Synonym
    HAI, HAI1, MANSC2
  • Source
    Biotinylated Mouse SPINT1 Protein, His,Avitag(SP1-M82E3) is expressed from human 293 cells (HEK293). It contains AA Glu 30 - Glu 443 (Accession # Q9R097).
    Predicted N-terminus: Glu 30
  • Molecular Characterization
    SPINT1 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 50 kDa. The protein migrates as 53-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
SPINT1 SDS-PAGE

Biotinylated Mouse SPINT1 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
SPINT1 MALS images

The purity of Biotinylated Mouse SPINT1 Protein, His,Avitag (Cat. No. SP1-M82E3) is more than 90% and the molecular weight of this protein is around 50-75 kDa verified by SEC-MALS.

  • Background
    The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured t.
  • Clinical and Translational Updates

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