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AAV8 Titration ELISA Kit

For research use only.
    Product Specifications
    Assay TypeSandwich-ELISA
    AnalyteAAV8
    Format96T
    Regulatory StatusRUO
    Sensitivity<1.75E+07capsids/mL
    Standard Curve Range5.60E+08 capsids/mL-1.75E+07 capsids/mL
    Assay Time3 hr 20 min
    Suitable Sample TypeFor the detection and quantitative determination of AAV8 capsid titration in AAV gene therapy product preparation processing.
    Sample volume100 uL
  • Product Overview
    AAV8 Titration ELISA Kit is based on the sandwich-ELISA method and is used to detect and quantitatively determine the AAV8 capsid titration in AAV gene therapy product winthin 3 hr 20 min. The kit is designed to provide reliable solutions for the early development, production, and quality control of AAV gene therapy products.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Materials Provided
    IDComponentsSize
    AAV08-C01Pre-coated Anti-AAV8 Antibody Microplate1 plate
    AAV08-C02AAV8 Standard1.4E+10 capsids
    AAV08-C03Biotin-Anti-AAV8 Antibody15 μg
    AAV08-C04Streptavidin-HRP50 μL
    AAV08-C0510×Washing Buffer 50 mL
    AAV08-C062×Dilution Buffer50 mL
    AAV08-C07Substrate Solution12 mL
    AAV08-C08Stop Solution7 mL
Typical Data Please refer to DS document for the assay protocol.
 AAV8 TYPICAL DATA

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

  • Background: AAV8
    The adeno-associated virus (AAV) is a small (25 nm), non-enveloped virus of the parvoviridae family, including 12 different AAV serotypes. In the parvoviridae family it belongs to the genus dependoparvovirus, because it needs the presence of a helper virus for replication and assembly. The icosahedral AAV capsid composed of the capsid proteins VP1, VP2 and VP3 contains a linear, single-stranded DNA genome of 4.7 kb.
  • Clinical and Translational Updates

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ETD of in-stock products: 4 business days

価格(USD) : 565.00

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