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Biotinylated Human latent GDF-8 Protein, His Tag, ultra sensitivity (primary amine labeling) (MALS & SPR verified)

  • Source
    Biotinylated Human latent GDF-8 Protein, His Tag, primary amine labeling(GD8-H8243) is expressed from human 293 cells (HEK293). It contains AA Asn 24 - Ser 375 (Accession # O14793).
    Predicted N-terminus: His
  • Molecular Characterization
    latent GDF-8 Structure

    The protein has a calculated MW of 42.0 kDa. The protein migrates as 38 kDa and 50-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 0.2 M Arginine, PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
latent GDF-8 SDS-PAGE

Biotinylated Human latent GDF-8 Protein, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
latent GDF-8 MALS images

The purity of Biotinylated Human latent GDF-8 Protein, His Tag, primary amine labeling (Cat. No. GD8-H8243) is more than 90% and the molecular weight of this protein is around 90-110 kDa verified by SEC-MALS.

Bioactivity-SPR
 latent GDF-8 SPR

Human Follistatin Protein, His Tag, premium grade (Cat. No. FON-H52H4) immobilized on CM5 Chip can bind Biotinylated Human latent GDF-8 Protein, His Tag (Cat. No. GD8-H8243) with an affinity constant of 248 nM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    Growth differentiation factor 8 (GDF8), also known as myostatin, is a unique member of the transforming growth factor-β superfamily that is expressed in human granulosa cells and has important roles in regulating a variety of ovarian functions. GDF8 acts as a negative regulator of skeletal muscle growth and differentiation. In addition to the expression in the musculoskeletal system, GDF8 is also expressed in various tissues, including the reproductive system.
  • Clinical and Translational Updates

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