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Your Position: ホーム > Protein > TSLP R > TSR-H82F3

Biotinylated Human TSLP R Protein, Fc,Avitag™

  • Synonym
    TSLP R,CRLF2,IL-XR,ILXR,p2RY8,CRLF2 fusion,TSLP receptor,TSLPR,CRL2,CRLF2Y,Cytokine receptor-like 2
  • Source
    Biotinylated Human TSLP R Protein, Fc,Avitag(TSR-H82F3) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Lys 231 (Accession # Q9HC73-1).
    Predicted N-terminus: Gly 25
  • Molecular Characterization
    TSLP R Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 52.1 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
TSLP R SDS-PAGE

Biotinylated Human TSLP R Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 TSLP R ELISA

Immobilized Biotinylated Human TSLP R Protein, Fc,Avitag (Cat. No. TSR-H82F3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human TSLP Protein (R127A, R130A), His Tag (Cat. No. TSP-H5243) with a linear range of 2-39 ng/mL (QC tested).

 TSLP R ELISA

Immobilized Human TSLP Protein (R127A, R130A), His Tag (Cat. No. TSP-H5243) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TSLP R Protein, Fc,Avitag (Cat. No. TSR-H82F3) with a linear range of 0.2-5 ng/mL (Routinely tested).

 TSLP R ELISA

Immobilized Cynomolgus TSLP, His Tag (Cat. No. TSP-C52H8) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TSLP R Protein, Fc,Avitag (Cat. No. TSR-H82F3) with a linear range of 0.6-20 ng/mL (Routinely tested).

  • Background
    Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells.
  • Clinical and Translational Updates

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