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ClinMax™ Human Soluble Siglec-2 / CD22 ELISA Kit, PRO

For research use only.
    Product Details
    ClinMax™ Human Soluble Siglec-2/CD22 ELISA Kit, PRO is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human Siglec-2/CD22 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
    1. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    2. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    3. Simplified and straightforward protocols and ready-to-use reagents to save assay time.
  • Application

    The kit is developed for quantitative detection of natural and recombinant human Siglec-2/CD22 in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow
     Siglec-2 Workflow
    Key Features
    AnalyteSiglec-2/CD22
    Assay TypeSandwich-ELISA
    ReactivityHuman
    Sensitivity<35.0 pg/mL
    Range93.75 pg/mL-6000 pg/mL
    Assay Time3hr
    Sample TypeCell Culture Supernatants, Plasma, Serum.
    Sample volume50 μL
    Format96-wells plate breakable into 12 x 8 wells strips

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Typical Data Please refer to DS document for the assay protocol.
 Siglec-2 TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of CD22 is less than 35 pg/mL.

Validation
Intra-Assay Statistics

Ten replicates of each of 4 samples containing different CD22 concentrations were tested in one assay. Acceptable criteria: CV<10%.

 Siglec-2 INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Four samples containing different concentrations of CD22 were tested in independent assays. Acceptable criteria: CV<15%.

 Siglec-2 INTER-ASSAY STATISTICS
Recovery

Recombinant CD22 was spiked into 3 human serum samples, and then analyzed. The average recovery of CD22 for serum samples is 105%.

 Siglec-2 RECOVERY
Materials Provided
IDComponentsSize
CEA046-C01Pre-coated Anti-Siglec-2/CD22 Antibody Microplate1 plate
CEA046-C02Human Siglec-2/CD22 Standard100 μg×2
CEA046-C03Biotin-Anti-Siglec-2/CD22 Antibody Con. Solution300 μL
CEA046-C04Biotin-Antibody Dilution Buffer8mL
CEA046-C05Streptavidin-HRP Con. Solution500 μL
CEA046-C06Streptavidin-HRP Dilution Buffer15 mL
CEA046-C0720× Washing Buffer50 mL
CEA046-C08Sample Dilution Buffer15 mL ×2
CEA046-C09Substrate Solution12 mL
CEA046-C10Stop Solution6 mL
  • Clinical and Translational Updates

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ETD of in-stock products: 4 business days

価格(JPY) : 139,440

価格(JPY) : 627,480

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