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resDetect™ T7 RNA Polymerase ELISA Kit (Residue Testing)

For research use only.
    Product Specifications
    Assay TypeSandwich-ELISA
    AnalyteT7 RNA polymerase
    Format96T(8×12 strips)
    ReactivityHuman
    Regulatory StatusRUO
    Sensitivity<0.78 ng/Ml
    Standard Curve Range0.78 ng/mL-50 ng/mL
    Assay Time3 hr 20 min
    Suitable Sample TypeFor the quantitative determination of T7 RNA polymerase in Cell Culture Supernatants.
    Sample volume100 uL
  • Product Overview
    resDetect™ T7 RNA Polymerase ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is designed to detect and quantitatively determine T7 RNA Polymerase residues in mRNA drugs. The kit contains T7 RNA Polymerase (ACROBiosystems, cat# T7E-E5143) to ensure accurate assay results and is designed to provide a reliable solution for monitoring and controlling T7 RNA Polymerase residues during the production of mRNA products. It can also be used as a universal detection tool for the quantitative determination of T7 RNA Polymerase.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Materials Provided
    IDComponentsSize
    RES018-C01Pre-coated Anti-T7 RNA polymerase Antibody Microplate1 plate(8×12 strips)
    RES018-C02T7 RNA polymerase Standard100 μL
    RES018-C03Biotin-Anti-T7 RNA polymerase Antibody150 μL
    RES018-C04Streptavidin-HRP50 μL
    RES018-C0520xWashing Buffer 50 mL
    RES018-C06Biotin-Antibody and Streptavidin-HRP Dilution Buffer50 mL
    RES018-C07Standard and Sample Dilution Buffer (5x)30 mL
    RES018-C08Substrate Solution12 mL
    RES018-C09Stop Solution7 mL
Typical Data Please refer to DS document for the assay protocol.
 T7 RNA polymerase TYPICAL DATA

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

Validation
Intra-Assay Statistics

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV<10%.

 T7 RNA polymerase INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<10%.

 T7 RNA polymerase INTER-ASSAY STATISTICS
Recovery

Three T7 RNA polymerases with different concentrations were tested to calculate the recovery rate.

 T7 RNA polymerase RECOVERY
  • Clinical and Translational Updates

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ETD of in-stock products: 4 business days

価格(USD) : 605.00

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