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Your Position: ホーム > Protein > CD7 > CD7-C82E8

Biotinylated Cynomolgus CD7 Protein, His,Avitag™ (MALS & SPR verified)

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    CD7,GP40,TP41,LEU-9,Tp40
  • Source
    Biotinylated Cynomolgus CD7 Protein, His,Avitag(CD7-C82E8) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Ser 177 (Accession # A0A2K5VA18).
    Predicted N-terminus: Ala 26
    Request for sequence
  • Molecular Characterization
    CD7 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 19.6 kDa. The protein migrates as 30-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD7 SDS-PAGE

Biotinylated Cynomolgus CD7 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CD7 MALS images

The purity of Biotinylated Cynomolgus CD7 Protein, His,Avitag (Cat. No. CD7-C82E8) is more than 90% and the molecular weight of this protein is around 43-53 kDa verified by SEC-MALS.

Report
Bioactivity-SPR
CD7 SPR

Biotinylated Cynomolgus CD7 Protein, His,Avitag (Cat. No. CD7-C82E8) immobilized on CM5 Chip can bind Human SECTM1, His Tag (Cat. No. SE1-H5227) with an affinity constant of 12.4 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Protocol
  • Background
    T-cell antigen CD7 (CD7) is also known as GP40, LEU-9, TP41 and Tp40. CD7 is a protein that in humans is encoded by the CD7 gene, this gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. CD7 has been shown to interact with PIK3R1. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
  • Clinical and Translational Updates

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