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Anti-SARS-CoV-2 Spike Antibody, Human IgG1 (9A5E10) (BA.4&BA.5/Omicron Specific) (MALS verified)

  • Source

    Anti-SARS-CoV-2 Spike Antibody, Human IgG1 (9A5E10) (BA.4&BA.5/Omicron Specific) is isolated from a Spike protein infected Mouse and is recombinantly produced from human 293 cells (HEK293)

  • Clone

    9A5E10

  • Isotype

    Human IgG1 | Human Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Virus

  • Immunogen

    Recombinant SARS-CoV-2 Spike Trimer Protein (BA.4/Omicron) erived from human 293 cells.

  • Specificity

    This product is a specific antibody specifically reacts with Spike protein.

  • Application
    ApplicationRecommended Usage
    ELISA0.2-50 ng/mL
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Spike protein SDS-PAGE

Anti-SARS-CoV-2 Spike Antibody, Human IgG1 (9A5E10) (BA.4&BA.5/Omicron Specific) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Spike protein MALS images

The purity of Anti-SARS-CoV-2 Spike Antibody, Human IgG1 (9A5E10) (BA.4&BA.5/Omicron Specific) (Cat. No. SPN-M551) is more than 90% and the molecular weight of this protein is around 130-150 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Spike protein ELISA

Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.4/Omicron) (Cat. No. SPN-C5229) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike Antibody, Human IgG1 (9A5E10) (BA.4&BA.5/Omicron Specific) (Cat. No. SPN-M551) with a linear range of 0.2-6 ng/mL (QC tested).

 Spike protein ELISA

Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.5/Omicron) (Cat. No. SPN-C522e) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike Antibody, Human IgG1 (9A5E10) (BA.4&BA.5/Omicron Specific) (Cat. No. SPN-M551) with a linear range of 0.2-6 ng/mL (QC tested).

 Spike protein ELISA

Immobilized Different SARS-CoV-2 Spike protein, His Tag at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike Antibody, Human IgG1 (9A5E10) (BA.4&BA.5/Omicron Specific) (Cat. No. SPN-M551) with a linear range of 0.1-13 ng/mL (Routinely tested).

  • Background
    It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates

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