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Human Coagulation factor IX / F9 Protein, His Tag (active enzyme, MALS verified)

  • Synonym
    Coagulation factor Ix,PTC,F9,THPH8,FIX,Plasma Thromboplastic Component,factor Ix
  • Source
    Human Coagulation factor IX Protein, His Tag(FIX-H52H3) is expressed from human 293 cells (HEK293). It contains AA Thr 29 - Thr 461 (Accession # P00740-1).
    Predicted N-terminus: Thr 29
  • Molecular Characterization
    Coagulation factor IX Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 50.6 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,pH8.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Coagulation factor IX SDS-PAGE

Human Coagulation factor IX Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Coagulation factor IX MALS images

The purity of Human Coagulation factor IX Protein, His Tag (Cat. No. FIX-H52H3) is more than 90% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2, The specific activity is 54-107 pmol/min/μg (QC tested).

  • Background
    Coagulation Factor IX is also known as F9, FIX, and P19. Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ ions, phospholipids, and factor VIIIa,and plays an important role in the intrinsic blood coagulation cascade. As a key component of the clotting cascade, Factor IX helps form stable blood clots and prevents excessive bleeding.
  • Clinical and Translational Updates

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