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ClinMax™ Human GM-CSF ELISA Kit

For research use only.
    Product Details
    ClinMax™ Human GM-CSF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human GM-CSF that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
    1. Standards to calibrate with NIBSC/WHO standards for comparable results.
    2. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    3. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    4. Simplified and straightforward protocols and ready-to-use reagents to save assay time.
  • Application

    The kit is developed for quantitative detection of natural and recombinant human GM-CSF in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow
     GM-CSF Workflow
    Key Features
    AnalyteGM-CSF
    Assay TypeSandwich-ELISA
    ReactivityHuman
    Sensitivity10 pg/mL
    Range12.5 pg/mL-1600 pg/mL
    Assay Time1.75 hr
    Sample TypeCell Culture Supernatants, Plasma, Serum.
    Sample volume50 uL
    Format96-wells plate breakable into 12 x 8 wells strips

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Storage
    Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Typical Data Please refer to DS document for the assay protocol.
 GM-CSF TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of GM-CSF is less than 10.0 pg/mL.

Validation
Intra-Assay Statistics

Ten replicates of each of 3 samples containing different GM-CSF concentrations were tested in one assay. Acceptable criteria: CV<10%.

 GM-CSF INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Three samples containing different concentrations of GM-CSF were tested in independent assays. Acceptable criteria: CV<15%.

 GM-CSF INTER-ASSAY STATISTICS
Recovery

Recombinant GM-CSF was spiked into 5 human serum samples, and then analyzed. The average recovery of GM-CSF for serum samples is 102.82%.

 GM-CSF RECOVERY
Materials Provided
IDComponentsSize
CRB007-C01Pre-coated Anti-GM-CSF Antibody Microplate1 plate
CRB007-C02Human GM-CSF Standard32 μg×2
CRB007-C03Biotin-Anti-GM-CSF Antibody Con. Solution100 μL
CRB007-C04Biotin-Antibody Dilution Buffer8 mL
CRB007-C05Streptavidin-HRP Con. Solution500μL
CRB007-C06Streptavidin-HRP Dilution Buffer15 mL
CRB007-C0720× Washing Buffer50 mL
CRB007-C08Sample Dilution Buffer15 mL×2
CRB007-C09Substrate Solution12 mL
CRB007-C10Stop Solution6 mL
  • Clinical and Translational Updates

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価格(JPY) : 139,440

価格(JPY) : 627,480

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