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Cynomolgus ALPP Protein, His Tag

  • Synonym
    PLAP
  • Source
    Cynomolgus ALPP, His Tag(ALP-C52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 21 - Asp 504 (Accession # XP_045223825.1).
    Predicted N-terminus: Ile 21
  • Molecular Characterization
    ALPP Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 54.6 kDa. The protein migrates as 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50mM Tris,150mM NaCl pH7.3 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
ALPP SDS-PAGE

Cynomolgus ALPP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Background
    In most mammals there are four different isozymes: placental (ALPP), germ cell (ALPG), intestinal (ALPI) and tissue non-specific (liver/bone/kidney) (ALPL/TP). ALPP is also known as Alkaline phosphatase, placental type and PLAP. Alkaline phosphatase that can hydrolyze various phosphate compounds.
  • Clinical and Translational Updates

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