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resDetect™ Human Interleukin-6 (IL-6) ELISA Kit (Residue Testing)

For research use only.
    Product Specifications
    Assay TypeSandwich-ELISA
    AnalyteIL-6
    Format96T(8×12 strips)
    ReactivityHuman
    Regulatory StatusRUO
    Sensitivity<2.34pg/mL
    Standard Curve Range2.34 pg/mL-150 pg/mL
    Assay Time2 hr 50 min
    Suitable Sample TypeFor the quantitative determination of human IL-6 in Cell Culture Supernatants, Plasma, Serum.
    Sample volume100 uL
  • Product Overview
    resDetect™ Human Interleukin-6 (IL-6) ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human IL-6 in cell culture supernatants, serum, and plasma. The kit contains GMP human IL-6 (ACROBiosystems, cat#GMP-L06H27) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of human IL-6.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Materials Provided
    IDComponentsSize
    CRS005-C01Pre-coated Anti-IL-6 Antibody Microplate1 plate(8×12 strips)
    CRS005-C02Human IL-6 Standard20 μg
    CRS005-C03Biotin-Anti-IL-6 Antibody50 μL
    CRS005-C04Streptavidin-HRP50 μL
    CRS005-C0510xWashing Buffer 50 mL
    CRS005-C062xDilution Buffer50 mL
    CRS005-C07Substrate Solution12 mL
    CRS005-C08Stop Solution7 mL
Typical Data Please refer to DS document for the assay protocol.
 IL-6 TYPICAL DATA

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

Validation
Dilution Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human IL-6 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

 IL-6 DILUTION LINEARITY
Intra-Assay Statistics

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV<10%.

 IL-6 INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<10%.

 IL-6 INTER-ASSAY STATISTICS
Recovery

Three parts of blank serum were added with different concentrations of human IL-6, and the serum without human IL-6 was used as background to calculate the recovery rate. The range of the recovery rate is 90.6-114.8%, and the average recovery is 101.2%.

 IL-6 RECOVERY
  • Background: IL-6
    IL6,Interleukin-6,BSF2,HSF,IFNB2
    Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells,et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimer's Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.
  • Clinical and Translational Updates

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