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Your Position: ホーム > Protein > Klotho beta > KLB-R52H8

Rhesus macaque Klotho beta / KLB Protein, His Tag

  • Synonym
    betaKlotho,beta-klotho,BKL,KLB,klotho beta like,klotho beta-like protein
  • Source
    Rhesus macaque Klotho beta, His Tag(KLB-R52H8) is expressed from human 293 cells (HEK293). It contains AA Phe 53 - Leu 997 (Accession # G7MSR8-1).
    Predicted N-terminus: Phe 53
  • Molecular Characterization
    Klotho beta Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 110.3 kDa. The protein migrates as 115-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Klotho beta SDS-PAGE

Rhesus macaque Klotho beta, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-BLI
 Klotho beta BLI

Loaded Human FGF R4, Fc Tag (Cat. No. FG4-H5253) on Protein A Biosensor, can bind Rhesus macaque Klotho beta, His Tag (Cat. No. KLB-R52H8) with an affinity constant of 0.9 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

 Klotho beta BLI

Loaded Human FGF19, Fc Tag (Cat. No. FG9-H5253) on Protein A Biosensor, can bind Rhesus macaque Klotho beta, His Tag (Cat. No. KLB-R52H8) with an affinity constant of 78.5 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

  • Background
    KLB (Klotho Beta) is a Protein Coding gene. Among its related pathways are RET signaling and HIV Life Cycle. GO annotations related to this gene include hydrolase activity, hydrolyzing O-glycosyl compounds and fibroblast growth factor binding. An important paralog of this gene is KL. Klotho Beta is a regulator in multiple metabolic processes, while its role in cancer remains unclear. We found the expression of βKlotho was down-regulated in human hepatocellular carcinoma tissues compared with that in paired adjacent non-tumourous liver tissues. Hepatoma cells also showed decreased expression of βKlotho compared with normal hepatocyte cells. Reintroduction of βKlotho into hepatoma cells inhibited their proliferation.
  • Clinical and Translational Updates

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