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Human MASP3 (450-721) Protein, His Tag (active enzyme)

  • Synonym
    MASP3, MASP1, CRARF1, RaRF
  • Source
    Human MASP3 (450-721), His Tag(MA3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 450 - Val 721 (Accession # P48740-2).
    Predicted N-terminus: Ile 450
  • Molecular Characterization
    MASP3 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 31.6 kDa. The protein migrates as 38-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
MASP3 SDS-PAGE

Human MASP3 (450-721), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB).The specific activity is >7500 pmol/min/µg,as measured under the described conditions (QC tested).

  • Background
    MASP3 is a member of the MASP family. The MASP family proteins were first
    discovered as complexes with mannose-binding lectin (MBL) and therefore named MBL-associated
    serine proteases, but later, they were found to interact with ficolins, and later still, collectin-10
    and collectin-11. As well as proteolytic enzymes (MASP-1, MASP-2, MASP-3), the group includes
    non-enzymatic factors (MAp19, MAp44). In this review, the association-specific factors of the lectin
    pathway with haematologic malignancies and related infections are discussed.
  • Clinical and Translational Updates

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