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Human Integrin alpha 10 beta 1 (ITGA10&ITGB1) Heterodimer Protein, His Tag&Tag Free

  • シノニム
    Integrin alpha 10 beta 1,ITGA10&ITGB1
  • ソース
    Human ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52Wa) is expressed from human 293 cells (HEK293). It contains AA Phe 23 - Ser 1122 (ITGA10) & Gln 21 - Asp 728 (ITGB1) (Accession # O75578-1 (ITGA10) & P05556-1 (ITGB1)).
    Predicted N-terminus: Phe 23 (ITGA10) & Gln 21 (ITGB1)
  • 分子特性
    Online(Phe 23 - Ser 1122 (ITGA10) & Gln 21 - Asp 728 (ITGB1)) O75578-1 (ITGA10) & P05556-1 (ITGB1)

    Human ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA10 and ITGB1, has a calculated MW of 126.6 kDa (ITGA10) and 83.7 kDa (ITGB1). Subunit ITGA10 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 140-160 kDa (ITGA10) and 100-130 kDa (ITGB1) respectively due to glycosylation.

  • エンドトキシン
    Less than 1.0 EU per μg by the LAL method.
  • 純度

    >95% as determined by SDS-PAGE.

  • 成分

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wa) SDS-PAGE gel

Human ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag FreeHuman ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wa) ELISA bioactivity

Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Human ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52Wa) with a linear range of 0.02-0.313 μg/mL (QC tested).

  • バックグラウンド
    Human integrin alpha(10)I domain as a recombinant protein to reveal its ligand binding specificity. In general, alpha(10)I did recognize collagen types I-VI and laminin-1 in a Mg(2+)-dependent manner, whereas its binding to tenascin was only slightly better than to albumin. Alpha 10 beta 1 is a known collagen-binding I domain integrin, in addition to α1β1, α2β1 and α11β1. GROGER found in the N-terminal domain of collagens I and III, is only weakly recognised by α10β1, an important collagen receptor on chondrocytes, contrasting with the other collagen-binding integrins.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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