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Your Position: ホーム > Protein > CTGF > CTF-M52Hc

Mouse CTGF / CCN2 Protein, His Tag

  • Synonym
    CCN2,NOV2,HCS24,IGFBP8,IBP-8,IGFBP-8,IGF-binding protein 8
  • Source
    Mouse CTGF, His Tag(CTF-M52Hc) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Ala 348 (Accession # P29268-1).
    Predicted N-terminus: Gln 26
  • Molecular Characterization
    CTGF Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 37.3 kDa. The protein migrates as 37-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Potassium glutamate and Arginine, pH7.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CTGF SDS-PAGE

Mouse CTGF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 CTGF ELISA

Immobilized Mouse CTGF, His Tag (Cat. No. CTF-M52Hc) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CTGF Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).

Bioactivity-BLI
 CTGF BLI

Loaded Monoclonal Anti-Human CTGF Antibody, Human IgG1 on AHC Biosensor, can bind Mouse CTGF, His Tag (Cat. No. CTF-M52Hc) with an affinity constant of 2.46 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Connective Tissue Growth Factor (CTGF), also known as CCN2, is a member of the CCN (CCN1-6) family of modular matricellular proteins. Like other CCN proteins, mature human CTGF consists of IGF-binding protein domain, a vWF-C domain, a TSP-1 domain, and a cysteine knot heparin-binding domain. CTGF promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Analysis of CCN2 function in vivo has focused primarily on its key role as a mediator of excess ECM synthesis in multiple fibrotic diseases.
  • Clinical and Translational Updates

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