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Biotinylated Human ROR2 / NTRKR2 Protein, His,Avitag™

  • シノニム
    ROR2,NTRKR2
  • ソース
    Biotinylated Human ROR2, His,Avitag (RO2-H82E3) is expressed from human 293 cells (HEK293). It contains AA Glu 34 - Gly 403 (Accession # A1L4F5-1).
    Predicted N-terminus: Glu 34
  • 分子特性
    Online(Glu 34 - Gly 403) A1L4F5-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 45.0 kDa. The protein migrates as 55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • ビオチン化
    ビオチン化 of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • エンドトキシン
    Less than 1.0 EU per μg by the LAL method.
  • 純度

    >95% as determined by SDS-PAGE.

  • 成分

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • 再構成

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 保存

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human ROR2, His,Avitag (Cat. No. RO2-H82E3) SDS-PAGE gel

Biotinylated Human ROR2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human ROR2, His,AvitagBiotinylated Human ROR2, His,Avitag (Cat. No. RO2-H82E3) ELISA bioactivity

Immobilized Biotinylated Human ROR2, His,Avitag (Cat. No. RO2-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-ROR2 Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (QC tested).

  • バックグラウンド
    Tyrosine-protein kinase transmembrane receptor ROR2 is also known as Neurotrophic tyrosine kinase, receptor-related 2 (NTRKR2), which belongs to the protein kinase superfamily and Tyr protein kinase family and ROR subfamily. ROR2 is a homodimer protein, which can binds YWHAB or interact with WTIP. ROR2 may be involved in the early formation of the chondrocytes. It seems to be required for cartilage and growth plate development.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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