ログイン 会員登録
icon_bulk_order一括注文 Acrobiosystems for English
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
phone
Your Position: ホーム > Protein > IL-7 R alpha > IL7-H5258

Human IL-7 R alpha / CD127 Protein (I66T, V138I), Mouse IgG2a Fc Tag

  • Synonym
    IL7Ra,CD127
  • Source
    Human IL-7 R alpha, Mouse IgG2a Fc Tag(IL7-H5258) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Gly 236 (Accession # P16871-1 (I66T,V138I)).
    Predicted N-terminus: Glu 21
  • Molecular Characterization
    IL-7 R alpha Structure

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 52.0 kDa. The protein migrates as 75-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-7 R alpha SDS-PAGE

Human IL-7 R alpha, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 IL-7 R alpha ELISA

Immobilized Human IL-7 Protein, premium grade (Cat. No. IL7-H4219) at 2 μg/mL (100 μL/well) can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. IL7-H5258) with a linear range of 4-63 ng/mL (QC tested).

 IL-7 R alpha ELISA

Biotinylated Human TSLP (R127A, R130A), His,Avitag (Cat. No. TSP-H82E0) immobilized at 2 μg/mL (100 μL/well) via precoated 5 μg/mL (100 μL/well) of Human TSLP R, Fc Tag (Cat. No. TSR-H525a), can bind can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. IL7-H5258) with a linear range of 1-39 ng/mL (Routinely tested).

Bioactivity-SPR
 IL-7 R alpha SPR

Captured Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. IL7-H5258) on CM5 chip via anti-mouse antibodies surface can bind ActiveMax® Human IL-7, Tag Free (Cat. No. IL7-H4219) with an affinity constant of 13.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

  • Background
    Interleukin-7 receptor subunit alpha (IL7Ra) is also known as CD antigen CD127, which belongs to the type I cytokine receptor family and type 4 subfamily. IL7Ra /CD127 contains 1 fibronectin type-III domain. IL7Ra /CD127 is expressed on various cell types, including naive and memory T cells and many others. IL7Ra /CD127 is receptor for interleukin-7 and also acts as a receptor for thymic stromal lymphopoietin (TSLP). The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R.
  • Clinical and Translational Updates

コメント (0)


ETD of in-stock products: 4 business days

価格(JPY) : 89,600

価格(JPY) : 646,800

プロモーション&展示



医薬品開発状況

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:9 Details
  • Latest Research Phase:Phase 2 Clinical

データシート&ドキュメンテーション

DMF請求
DMF (Drug Master File)

新製品

お問い合わせ&コメント

This web search service is supported by Google Inc.

totopphone