ログイン 会員登録
icon_bulk_order一括注文 Acrobiosystems for English
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
phone
Your Position: ホーム > Protein > PCSK9 > PC9-H52E4

Hamster PCSK9 Protein, His Tag

  • Synonym
    PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
  • Source
    Hamster PCSK9, His Tag(PC9-H52E4) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Ser 691 (Accession # G3GTK5).
    Predicted N-terminus: Gln 30
  • Molecular Characterization
    PCSK9 Structure

    This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.9 kDa and 59.0 kDa respectively. The protein migrates as 18 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
PCSK9 SDS-PAGE

Hamster PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 PCSK9 ELISA

Immobilized Human LDL R, Fc Tag at 5 μg/mL (100 μL/well) can bind Hamster PCSK9, His Tag (Cat. No. PC9-H52E4) with a linear range of 0.078-0.313 μg/mL (QC tested).

 PCSK9 ELISA

Immobilized Hamster PCSK9, His Tag (Cat. No. PC9-H52E4) at 1 μg/mL (100 μL/well) can bind Evolocumab with a linear range of 2-63 ng/mL (Routinely tested).

Bioactivity-BLI
 PCSK9 BLI

Loaded Human LDL R, Fc Tag on Protein A Biosensor, can bind Hamster PCSK9, His Tag (Cat. No. PC9-H52E4) with an affinity constant of 4.09 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
  • Clinical and Translational Updates

コメント (0)


ETD of in-stock products: 4 business days

価格(JPY) : 72,800

価格(JPY) : 610,400

プロモーション&展示



医薬品開発状況

  • Number of Launched Drugs:4 Details
  • Number of Drugs in Clinical Trials:27 Details
  • Latest Research Phase:Approved

データシート&ドキュメンテーション

DMF請求
DMF (Drug Master File)

関連製品一覧

新製品

お問い合わせ&コメント

This web search service is supported by Google Inc.

totopphone