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Human LBP / Lipopolysaccharide Protein, His Tag

  • Synonym
    Lipopolysaccharide-binding protein,LBP
  • Source
    Human Lipopolysaccharide, His Tag (LBP-H52H5) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Val 481 (Accession # P18428-1(I465F)).
    Predicted N-terminus: Ala 26
  • Molecular Characterization
    Online(Ala 26 - Val 481) P18428-1 (I465F)

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 52.8 kDa. The protein migrates as 62 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Lipopolysaccharide, His Tag (Cat. No. LBP-H52H5) SDS-PAGE gel

Human Lipopolysaccharide, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Lipopolysaccharide-binding protein (LBP),a member of the BPI/LBP/Plunc superfamily, BPI/LBP family, is detected in blood serum. LBP plays a role in the innate immune response. Alao,LBP can bind to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and act as an affinity enhancer for CD14, facilitating its association with LPS. Furthermore, LBP is able to promote the release of cytokines in response to bacterial lipopolysaccharide.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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