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Human Arginase 1 / ARG1 Protein, His Tag

  • Synonym
    ARG1,Arginase-1
  • Source
    Human Arginase 1, His Tag(AR1-H5228) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Lys 322 (Accession # NP_000036).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    Arginase 1 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 35.8 kDa. The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Arginase 1 SDS-PAGE

Human Arginase 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Arginase-1 (ARG1) is also known as Liver-type arginase, Type I arginase, which belongs to the arginase family. Arginase-1 / ARG1 is a manganese-containing enzyme. The reaction catalyzed by this enzyme is: arginine + H2O → ornithine + urea. It is the final enzyme of the urea cycle. Defects in Arginase-1 / ARG1 are the cause of argininemia (ARGIN).
  • Clinical and Translational Updates

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