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Your Position: ホーム > Protein > B7-1 > CD0-M5228

Mouse B7-1 / CD80 Protein, His Tag (MALS verified)

  • Synonym
    CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7
  • Source
    Mouse B7-1, His Tag(CD0-M5228) is expressed from human 293 cells (HEK293). It contains AA Val 38 - Lys 245 (Accession # Q00609-1).
    Predicted N-terminus: Val 38
  • Molecular Characterization
    B7-1 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 25.6 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
B7-1 SDS-PAGE

Mouse B7-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
B7-1 MALS images

The purity of Mouse B7-1, His Tag (Cat. No. CD0-M5228) is more than 85% and the molecular weight of this protein is around 35-50 kDa verified by SEC-MALS.

Bioactivity-ELISA
 B7-1 ELISA

Immobilized Mouse B7-1, His Tag (Cat. No. CD0-M5228) at 2 μg/mL (100 μL/well) can bind Mouse CTLA-4, Fc Tag (Cat. No. CT4-M5256) with a linear range of 0.1-2 ng/mL (QC tested).

  • Background
    B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response.
    B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
  • Clinical and Translational Updates

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価格(JPY) : 61,600

価格(JPY) : 369,600

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