Limited Edition Golden Llama is here! Check out how you can get one. Limited Edition Golden Llama is here! Check out how you can get one.
Offering SPR-BLI Services - Proteins provided for free! Offering SPR-BLI Services - Proteins provided for free!
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Here come GMP Grade Cytokines!Free Sample is available! Here come GMP Grade Cytokines!Free Sample is available!
Universality - Suitable for detection of natural or structurally conserved recombinant forms of Protein A and alkaline-resistant Protein A variants, such as MabSelect SuRe™ and other ligands
Fast time to results - less than 2 hours
Accuracy - Tracebility of Protein A standards against BSA China National Standard (NIFDC code: 140619) with validated pharmacopoeia quantitation methodology
Extensive validation: Validation Report (ICH compliant) available on request
High sensitivity - Sensitivity < 20 pg/mL of recombinant Protein A and MabSelect™ SuRe Protein A or other Protein A ligands
High IgG tolerance - Accurately quantify protein A in up to 10 mg/mL antibody
Excellent buffer compatibility
Assay Type | Sandwich-ELISA |
Analyte | Protein A |
Format | 96T |
Regulatory Status | RUO |
Sensitivity | < 20pg/mL |
Standard Curve Range | 25 pg/mL-1600 pg/mL |
Assay Time | 2 hr |
Suitable Sample Type | For the quantitative determination of recombinant Protein A, alkaline-resistant Protein A and MaXtar® ARPA ligand Protein A. |
Sample volume | 50 μL |
ID | Components | Size |
RES024-C01 | Pre-Coated Anti-Protein A Antibody Microplate | 1 plate |
RES024-C02A | Alkali-Tolerant Recombinant Protein A Standard (1μg/mL) | 100 μL |
RES024-C02B | MaXtar® ARPA ligand Protein A Standard (Bio-Link Co.) (1μg/mL) | 100 μL |
RES024-C03 | Recombinant Protein A Standard (1μg/mL) | 100 μL |
RES024-C04 | Biotin-Anti-Protein A Antibody | 700 μL |
RES024-C05 | Streptavidin-HRP | 300 μL |
RES024-C06 | Dilution Buffer | 100 mL |
RES024-C07 | 20×Washing Buffer | 30 mL |
RES024-C08 | Substrate Solution | 12 mL |
RES024-C09 | Stop Solution | 6 mL |
The Universal Protein A ELISA Kit can detect protein A or unnatural protein A variants within 2 hours, it is high sensitive and easy to use. Whether in upstream small-scale trials or downstream large-scale of antibody production processes, this kit can help you to accurate analysis of samples, monitor the protein A levels and ensure product quality.
The kit is developed for the detection of natural or structurally conserved recombinant forms of Protein A and alkaline-resistant Protein A variants, such as MabSelect SuRe™ , MaXtar® ARPA ligand (Bio-Link Co.), etc. in bioprocess manufacturing applications. It is used as a universal protein A and variants ligand detection tool to aid in optimal antibody purification process development and in routine quality control of in-process streams as well as final product.
It is for research use only.
Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Detection of Recombinant Protein A by sandwich-ELISA Assay.
Immobilized Anti-Protein A Antibody can bind Recombinant Protein A. Detection was performed using Biotin-Anti-Protein A Antibody with sensitivity of 25 pg/mL (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Detection of Alkali-Tolerant Recombinant Protein A by sandwich-ELISA Assay.
Immobilized Anti-Protein A Antibody can bind Alkali-Tolerant Recombinant Protein A. Detection was performed using Biotin-Anti-Protein A Antibody with sensitivity of 25 pg/mL (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Detection of MaXtar® ARPA ligand Protein A (Bio-Link Co.) by sandwich-ELISA Assay.
Immobilized Anti-Protein A Antibody can bind MaXtar® ARPA ligand Protein A (Bio-Link Co.). Detection was performed using Biotin-Anti-Protein A Antibody with sensitivity of 25 pg/mL (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision, Intra-Assay Precision CV<10%.
Three samples of known concentration were tested in ten separate assays to assess inter-assay precision, Inter-Assay Precision CV<20%.
The following recovery tests were conducted by adding different concentrations of Protein A to Human IgG1(Bevacizumab), and the final concentration of Human IgG1(Bevacizumab)is 10mg/mL to calculate the recovery rate. For Recombinant Protein A, Alkali-Tolerant Recombinant Protein A and MaXtar® ARPA ligand Protein A (Bio-Link Co.), the recovery ranges are 98-118%, 86-109% and 79-106%, respectively.
The following recovery tests were conducted by adding different concentrations of Protein A to Human IgG4(Toripalimab), and the final concentration of Human IgG4(Toripalimab)is 10mg/mL to calculate the recovery rate. For Recombinant Protein A, Alkali-Tolerant Recombinant Protein A and MaXtar® ARPA ligand Protein A (Bio-Link Co.), the recovery ranges are 97-106%, 93-104% and 86-105%, respectively.
We have conducted interference effect test about frequently-used buffers, they have excellent buffer compatibility. For specific buffers, it is recommended that you verify recovery to determine the minimum dilution ratio.
Host cell protein (HCP) and host cell DNA (HCD) were added to human IgG1 (Bevacizumab) and human IgG4 (Toripalimab), respectively, which were higher than the usual quality standard limit. Then three samples of known concentration of Protein A were added, respectively, and the ratio of Protein A recovery in the Protein A added samples without HCP and HCD was added as the specificity verification index. The calculation formula was as follows: (S3-S1) / (S2-S1) × 100%, the experimental design is as follows.
The specificity results are as follows.
ACROBiosystemsは、二重特異性抗体の治療薬への臨床開発を加速するために、高い生物活性を持つ均一なCD3δ/CD3εおよびCD3γ/CD3εタンパク質のシリーズを開発しました。
Organoid Toolbox は、即使用可能のオルガノイド、オルガノイド分化ツールキット、創薬プロジェクトの進行を加速する、さまざまなサービスを含むオルガノイドソリューションのことです。
抗体の特性評価法を可能にするため、ACROBiosystemsは、IdeS、SpeB、EndoH、Endo Sプロテアーゼなどの一連の酵素を開発し、抗体とその関連する翻訳後修飾(PTM)の特性評価をサポートします。
IL-15、IL-7、IL-21など、高品質のGMP準拠サイトカイン製品を提供しており、免疫細胞治療薬の臨床研究をサポートし、医薬品規制当局の承認を加速できます。
注目されている50以上のCAR-Tターゲットがあり、CARの発現の検出のために設計された抗原タンパク質をフローサイトメトリーで検証し、CARの発現が高い特異性で検出できます。ロット間の一貫性が保証されています。また、PE / FITC標識タンパク質を利用することで、ワンステップ染色でCARの発現を高い特異性でバックグラウンドなしに検出できます。
GMP準拠サイトカイン、高品質の細胞活性化/増幅試薬、遺伝子改変試薬/酵素、CAR検出試薬などの製品を提供し、細胞、遺伝子治療用に全体的なソリューションを提供し、創薬から臨床研究までのすべての段階をサポートします。
CD20、Claudin18.2、CD133、GPRC5D、CCR5、CCR8などの安定で高活性な全長型膜貫通タンパク質は、免疫学、ELISA、SPR、BLI、細胞実験、CAR陽性率検出に利用できます。VLP、界面活性剤、ナノディスクなどのテクニカルプラットフォームによって複数回膜貫通タンパク質の医薬品創薬を促進できます。
これまでに知られている免疫チェックポイント分子をほぼ製品化しており、様々なタグの製品群を提供できます。天然高分子の構造はMALSによって、生物活性はELISA/SPR/BLI/FACSなどによって検証済みです。またBiotin/FITCなどの標識も選択でき、抗体のハイスループットスクリーニングに利用できます。
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すべての分子のFc受容体タンパク質だけでなく、一般的な変異体やビオチン標識タイプも含まれております。お客様のモノクローナル抗体の開発をサポートします。
インターロイキン、成長因子、ケモカイン、TNF など、様々なサイトカインターゲットの自然なコンフォメーションを確保するために HEK293 によって発現されます。SDS-PAGE/HPLC/SEC-MALS によって高純度は確認され、高い生物活性は ELISA/SPR/BLI によって確認されています。
AneuroはACROBiosystemsが神経科学研究のために設計した製品群のブランドです。神経科学研究を推進するための治療・診断用研究タンパク質、PFF、組み換え神経因子など、高品質の重要なタンパク質を提供しております。
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