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Biotinylated Mouse Carbonic Anhydrase XII Protein, His,Avitag™ (active enzyme, MALS verified)

  • Synonym
    Carbonic Anhydrase XII,CA XII,Car12,CA-XII,Carbonate dehydratase XII
  • Source
    Biotinylated Mouse Carbonic Anhydrase XII Protein, His,Avitag(CAI-M82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Ser 301 (Accession # Q8CI85-1).
    Predicted N-terminus: Ala 25
  • Molecular Characterization
    Carbonic Anhydrase XII Structure

    This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 35.0 kDa. The protein migrates as 42-47 kDa under reducing (R) condition (SDS-PAGE).

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, PH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Carbonic Anhydrase XII SDS-PAGE

Biotinylated Mouse Carbonic Anhydrase XII Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Carbonic Anhydrase XII MALS images

The purity of Biotinylated Mouse Carbonic Anhydrase XII Protein, His,Avitag (Cat. No. CAI-M82E3) is more than 90% and the molecular weight of this protein is around 38-48 kDa verified by SEC-MALS.

Bioactivity

Measured by its esterase activity. The specific activity is >50 pmol/min/μg (QC tested).

  • Background
    This gene encodes a membrane-bound member of the alpha carbonic anhydrase family of enzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. These proteins participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Alternative splicing results in multiple transcript variants.
  • Clinical and Translational Updates

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