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Expression analysis of human Transferrin on HEK293/Human Transferrin Stable Cell Line by FACS.
Cell surface staining was performed on HEK293/Human Transferrin Stable Cell Line or negative control cell using PE-labeled anti-human Transferrin antibody.
Biotinylated Human Transferrin Protein, His,Avitag (Cat. No. TRN-H82E3) captured on Biotin CAP-Series S Sensor Chip can bind Human Transferrin R, His Tag (Cat. No. CD1-H5243) with an affinity constant of 0.527 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Immobilized Biotinylated Human Transferrin R Protein, Fc,Avitag (Cat. No. TFR-H82F3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Biotinylated Human Transferrin R Protein, Fc,Avitag (Cat. No. TRN-H52H3) with a linear range of 5-78 ng/mL (QC tested).
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