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Your Position: ホーム > Kits > Th1/Th2 cytokine > FCM-C05R

ClinMax™ Human Th1/Th2 Cytokine Kit (Flow Cytometry Multiplex Bead Assay)

For research use only.
    Product Show

    Th1/Th2 cytokine PRODUCT SHOW

  • Application

    The Human Th1/Th2 Cytokine Kit can be used to quantitatively measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-6 (IL-6), Interleukin-10 (IL-10), Tumor Necrosis Factor-α (TNF-α), and Interferon-γ (IFN-γ) protein levels in a single sample.The kit performance has been optimized for analysis of specific cytokines in tissue culture supernatants, EDTA plasma, and serum samples.

    It is for research use only.

  • Background
    T helper cells (Th) play a vital role in the adaptive immune system by suppressing or regulating immune responses. After Th actived, it will differentiate into T-helper 1 (Th1) or T-helper 2 (Th2) effector cells. These two subsets are characterized by their distinct cytokine expression pattern and the immune function they mediate. Th1 and Th2 cells each produce a specialized combination of cytokines that depends on type of pathogen that the helper T cell has recognized-some cytokines are more effective than others in the process of eliminating certain invaders. In general, TH1 cytokines play the role of immune killing, while Th2 cytokines play the role of immune protection. Th1/Th2 paradigm provides rationale for developing new vaccines against infectious diseases and novel strategies for the therapy of allergic and autoimmune disorders.
  • Assay Principles
    Human Th1/Th2 Cytokine 6-plex panel is a multiplex bead-based indirect immunofluorescence assay, utilizing flow cytometry for data acquisition and classification. The beads conjugated with specific antibody, target protein (IL-2/4/6/10, TNF-α, IFN-γ) in specimens and PE-conjugated detection antibody form a sandwiches complex as bead-analyte-detection antibody. The intensity of PE fluorescence signal in proportion to the target protein in specimens, are assessed by flow cytometry.The present assay has been optimized for quntitatively analysis for the IL-2/4/6/10, TNF-α, IFN-γ in cell culture supernatant, plasma, and serum samples.
    Product Details
    Assay TypeFACS
    AnalyteIFN-γ, TNF-α, IL-10, IL-6, IL-4, IL-2
    Format96T
    ReactivityHuman
    Regulatory StatusRUO
    Sensitivity1.77pg/ml (IFN-γ), 0.76pg/ml (TNF-α), 0.27pg/ml (IL-10), 0.78pg/ml ( IL-6), 0.65pg/ml (IL-4), 0.70pg/ml (IL-2)
    Standard Curve Range9.8 ~ 10000pg/mL
    Assay Time3 hr
    Suitable Sample Typehuman serum, human plasma, cell culture supernatants
    Sample volume10 uL
    Materials Provided
    IDComponentsSize
    FCM05-C01Calibrator5ug(for each)×2
    FCM05-C02AHuman IL-2 Capture Beads96 tests
    FCM05-C02BHuman IL-4 Capture Beads96 tests
    FCM05-C02CHuman IL-6 Capture Beads96 tests
    FCM05-C02DHuman IL-10 Capture Beads96 tests
    FCM05-C02EHuman TNF-α Capture Beads96 tests
    FCM05-C02FHuman IFN-γ Capture Beads96 tests
    FCM05-C032 × Assay Buffer40 mL
    FCM05-C0410 × Wash Buffer10 mL
    FCM05-C05Detection antibody0.5 mL
    FCM05-C0696-well V-bottom plate1 plate
    FCM05-C0796-well Sealing film2 pieces
    FCM05-C08APC Positive Control0.5 mL
    FCM05-C09PE Positive Control0.5 mL
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

FACS Data Please refer to DS document for the assay protocol.
 Th1/Th2 cytokine FACS

A representative scatter plot showing single bead population in P1 gate (left), the beads conjugated with analyte-sepcific antibody differntiated by APC fluorescence intensity (middle), and fluorescence signal in propotion to concentration of the analyte was measured for each cytokine in PE channel (right). Data was generated on Beckman Cytoflex S Flow Cytometer, equipped with blue laser (488 nm) and red laser (640 nm).

Typical Data Please refer to DS document for the assay protocol.
 Th1/Th2 cytokine TYPICAL DATA

A double logarithmic fitting curve was plotted for each cytokine( IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ). The present curve of each cytokines was ranging from 9.8 pg/ml to 10000 pg/ml respectively .

  • Clinical and Translational Updates

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ETD of in-stock products: 4 business days

価格(USD) : 1500.00

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